You're probably thinking of the "Program initialization complete" signal, which you can define if you want the processor to wait to start the next program for your cue.? If you don't define it, or it doesn't go high for 60? seconds, the processor goes ahead and starts the next program anyway.
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I never add a signal, unless you are doing something where you want to stagger the startups or something due to something you are doing in Slot1 for example, reading a config file and then starting up the next slot(s).
I believe I heard somewhere that if you don't have a signal on those ports, the processor will sequentially start all programs that are loaded in order from slot1 to 10 automatically.? Because of this, I have not had to manually start slots before, and just let the automatic method do it.? This would be the normal/default behavior, and probably the most used case of 98% of programs out there.
And I believe if you have a signal defined there, then you would need to manually start the program slot that has the signal defined there.? (I may be wrong on this, but I think that's how it works).
Therefore, unless you have a good reason to manually start programs in slots (either out of order, or based on other logic), then leaving those undefined, with no signal on them, is the best choice, as then the processor loads the slots normally from 1 to 10 in order, one after another until all programs are started and running.