I am not sure what is going on here. I was originally having trouble connecting with the TinySA on my primary ham radio computer and took it over to another computer and had success. I went back to the first computer and reduced the number of transmit and receive buffers in the USB serial device driver and then had success on the first computer. The next day, I was back to square one with the TinySA app on both computers. I also was not able to see the TinySA in DfuSeDemo on either computer. Both computers are older systems running 32-bit Windows 10.
I then installed the DfuSeDemo application and the firmware update on a newer 64-bit Windows 10 computer and was able to update the firmware successfully. I also had no problems with the TinySA app on this newer computer. Apparently then the USB cable and connectors are OK as is the TinySA itself (it passed the self-test before and after the firmware update). The two older 32-bit computers are current with Windows 10 updates.