And, are you seeing the red 'PTT' LED on the SignaLink light up during the latter part of the "Auto Tune" sequence, and is the transmitter keying on your DR-185?
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When using the SignaLink for VARA FM, it isn't unusual fo the PTT LED not to light up for the initial steps of the "Auto Tune" sequence. Also, the "Auto Tune" sequence is a series of data bursts at increasing audio levels. If the transmit audio level is set too high, most transceivers will unkey the transmitter at the highest levels, at the end of the sequence, regardless of the PTT signal being present.
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If the gateway station doesn't receive the bursts at the end of the "Auto Tune" sequence, it won't transmit any acknowledgement message.
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Mark - AD7EF