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I have updated the firmware on my nanoVNA-H4 hardware build 4.3 using the NanoVNA-H.v1.2.40.bin file and STM32cube software, and notice that

the red battery LED no longer lights when charging or unit is turned on. Same effect after I upgraded my TinySA Ultra....no longer red LED function.

Is that normal?

If I read your post correctly,
"nanoVNA-H4 hardware build 4.3 using the NanoVNA-H.v1.2.40.bin "
Your problem is you used the WRONG firmware!!!
You can not use "H" firmware on a "H4"!!!!!
And expect it to work correctly.
They are two different NVA's
First, download and install the Firmware for the NanoVNA H4, then try it.
Clyde KC7BJE

Clyde,
THANK YOU!!! I did not catch the difference earlier, used the H4 firmware and all is working well now!
Thank you.
Bill WB6YVK