Tim,
Did you perhaps connect your PR3 to a different USB port on your computer? That will cause Windows to change the COM Port "number". Usually, plugging the PR3 into the original USB port will return to the "old" COM Port "number".
When the COM Port number changes, it is usually possible to find the new COM Port number using JMRI's "Preferences" tool, in the "Connection" tab. Typically, JMRI will show the current selection, in red (because JMRI cannot communicate with that port number), and a list of other possible numbers, in black. Usually, one of the other COM Port numbers is the correct one...
If that fails, there is always using the Windows "Device Manager" to figure out exactly which COM Port "number" is assigned to the PR3.
Regards,
Billybob