In October 2022, PacBio announced the Revio System. ?This is capable of delivering HiFi reads with lengths of 15000-18000 bases for under $1000 with 30x coverage depth on a human-sized genome. ?These are the same types of reads we see used in the ?related projects to assemble the gapless references such as CHM13.
From the product sheet it also looks like the call accuracy for variant callingis quite similar to the much shorter 150 base reads in Illumina NovaSeq like with Big Y 700.
The platform looks to be coming soon from Dante Labs, which as everyone should be aware has lead the market with getting prices down on WGS but also has notorious history with actually delivering in a timely fashion. ?I have one on order and will share my experiences. ?Best case scenario is that we will wind up with a gapless S1121 Y chromosome to use as a reference going forward. ?Worst case... I wind up with more garbage like the Oxford Nanopore Test they botched for me in 2019 and spending another 6 months getting a refund.
James
